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45-964 (3.8ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-964 (3.8ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

NFATC4 Antibody

CATALOG NUMBER: 45-964

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig
Immunogen:
The immunogen for this antibody is: C-SGFPAPPGEEPPA
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:No signal obtained yet but low background observed in MOLT-4, Jurkat, A431, HeLa, Human Brain and Human Heart lysates at upto 2ug/ml.Immunohistochemistry:In paraffin embedded Human Placenta shows weak nuclear staining in trophoblasts.. Recommended concentration, 2.5-3.8ug/ml.
Specificity:
This antibody is expected to recognise isoform 2, 3, 4 and 5 (NP_004545.2; NP_001185894.1; NP_001185895.1; NP_001185896.1 respectively).
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
NFATC4
Additional Names:
NFATC4, NFAT3, NF-ATc4, nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4, T cell transcription factor NFAT3, nuclear factor of activated T-cells, cytoplasmic 4
Protein Accession Number:
NP_004545.2,NP_001185894.1,NP_001185895.1,NP_001185896.1
Protein Gi Number:
27886563
Ncbi Gene Id Number:
4776
Background Reference 1:
Hoey T, Sun YL, Williamson K, Xu X. Isolation of two new members of the NF-AT gene family and functional characterization of the NF-AT proteins. Immunity 1995 May;2(5):461-72

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-964

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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