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Western blot analysis of anti-NFE2L2 Antibody in Ramos cell line lysates (35ug/lane)
Western blot analysis of Nrf2(Nfe2l2) using rabbit polyclonal Nrf2(Nfe2l2) Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Nrf2(Nfe2l2) gene (Lane 2).
Western blot analysis of anti-NFE2L2 Antibody in Ramos cell line lysates (35ug/lane)

NFE2L2 Antibody

CATALOG NUMBER: 62-788

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This NFE2L2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-59 amino acids from the N-terminal region of human NFE2L2.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
68 kDa
Purification:
This antibody is purified through a protein G column, followed by dialysis against PBS.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NFE2L2
Additional Names:
Nuclear factor erythroid 2-related factor 2, NF-E2-related factor 2, NFE2-related factor 2, HEBP1, Nuclear factor, erythroid derived 2, like 2, NFE2L2, NRF2
Protein Accession Number:
Q16236
Protein Gi Number:
25453452
Ncbi Gene Id Number:
4780
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NFE2 (MIM 601490), NFE2L1 (MIM 163260), and NFE2L2 comprise a family of human basic leucine zipper (bZIP) transcription factors. They share highly conserved regions that are distinct from other bZIP families, such as JUN (MIM 165160) and FOS (MIM 164810), although remaining regions have diverged considerably from each other (Chan et al., 1995).
Background Reference 1:
Zhao,X., Stroke 38 (12), 3280-3286 (2007) Li,M.H., J. Biol. Chem. 282 (39), 28577-28586 (2007)

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-788

List Size:
400 ul

List Price:

$495.00

Datasheet
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