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Antibody used in WB on Human 721_B at 0.2-1 ug/ml.
Antibody used in WB on Human 721_B at 0.2-1 ug/ml.

NFIC Antibody

CATALOG NUMBER: 27-242

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NFIC.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
NFIC antibody can be used for detection of NFIC by ELISA at 1:62500. NFIC antibody can be used for detection of NFIC by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
721_B Cell Lysate
Predicted Molecular Weight:
55 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store NFIC antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
NFIC
Additional Names:
NFIC, CTF, CTF5, MGC20153, NF-I, NFI
Protein Accession Number:
NP_995315
Protein Gi Number:
45505151
Ncbi Gene Id Number:
4782
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Brg- or hBrm-associated factor (BAF) complexes, a chromatin-remodeling complex family of mammalian cells, facilitate transcriptional activity by remodeling nucleosome structure. Brg1 is the core subunit of Brg-associated factor complexes. BAF complexes can interact with NF1/CTF and RNAP II, and this interaction is closely dependent on the activation of gene transcription.
Background Reference 1:
Hebert, S.L., Biochim. Biophys. Acta 1769 (11-12), 649-658 (2007).

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-242

List Size:
100 ul

List Price:

$519.00

Datasheet
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