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Antibody used in WB on Transfected 293T at 1.25 ug/ml.
Antibody used in WB on Transfected 293T at 1.25 ug/ml.

NFIL3 Antibody

CATALOG NUMBER: 28-908

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NFIL3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
NFIL3 antibody can be used for detection of NFIL3 by ELISA at 1:312500. NFIL3 antibody can be used for detection of NFIL3 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Tranfected 293T Cell Lysate
Predicted Molecular Weight:
51 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store NFIL3 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
NFIL3
Additional Names:
NFIL3, E4BP4, IL3BP1, NFIL3A, NF-IL3A
Protein Accession Number:
NP_005375
Protein Gi Number:
52630429
Ncbi Gene Id Number:
4783
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NFIL3 is a basic leucine zipper (bZIP) transcription factor that represses or activates transcription in non-osteoblastic cells. NFIL3 play a role in attenuation of PTH target gene transcription in osteoblasts. NFIL3 repression domain interacts specifically with the TBP binding repressor protein Dr1.Glucocorticoid-mediated upregulation of the bZIP transcriptional repressor gene, NFIL3, is dependent on [Ca2+]i levels, and correlates with Glucocorticoid-evoked apoptosis of Glucocorticoid-sensitive CEM-C7-14 cells. NFIL3 is involved in a distinct growth factor-regulated signaling pathway that is responsible for the survival of early B-cell progenitors, and whose alteration by E2A-HLF leads to childhood B lineage leukemia.
Background Reference 1:
Ozkurt, I.C., et al., (2003) J. Biol. Chem. 278 (29), 26803-26809.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-908

List Size:
100 ul

List Price:

$473.00

Datasheet
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