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45-965 staining (1ug/ml) of Jurkat nuclear cell lysate (RIPA buffer, 20ug total protein per lane). Detected using chemiluminescence.
45-965 Flow cytometric analysis of paraformaldehyde fixed Human peripheral blood mononuclear cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmun
45-965 staining (1ug/ml) of Jurkat nuclear cell lysate (RIPA buffer, 20ug total protein per lane). Detected using chemiluminescence.

NFIL3 Antibody

CATALOG NUMBER: 45-965

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-RLIATQPISASDSG
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx. 60kDa band observed in nuclear lysates of cell line Jurkat and HeLa (calculated MW of 51.5kDa according to NP_005375.2). The observed molecular weight corresponds to different antibodies from other commercial sources. An additional band of 30kDa was observed in lysates of cell line HeLa, which was completely blocked with the immunizing peptide. Recommended concentration: 0.5-2ug/ml. Primary incubation 1 hour at room temperature.Flow Cytometry: Flow cytometric analysis of Human peripheral blood mononuclear cells. Recommended concentration: 10ug/ml.
Positive Control 1:
Cat. No. 1224 - Daudi Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
NFIL3
Additional Names:
NFIL3, nuclear factor, interleukin 3 regulated, E4BP4, IL3BP1, NFIL3A, NF-IL3A, interleukin-3 promoter transcriptional activator, OTTHUMP00000021633
Protein Accession Number:
NP_005375.2
Protein Gi Number:
52630429
Ncbi Gene Id Number:
4783
Background Reference 1:
Zhang W, Zhang J, Kornuc M, Kwan K, Frank R, Nimer SD. Molecular cloning and characterization of NF-IL3A, a transcriptional activator of the human interleukin-3 promoter. Mol Cell Biol 1995 Nov;15(11):6055-63

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-965

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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