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Antibody used in IHC on mouse hippocampus at 1:200.
Antibody used in IHC on Human Prostate at 5.0 ug/ml.
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.
Antibody used in IHC on mouse hippocampus at 1:200.

NFIX Antibody

CATALOG NUMBER: 27-679

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NFIX.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
NFIX antibody can be used for detection of NFIX by ELISA at 1:312500. NFIX antibody can be used for detection of NFIX by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
49 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store NFIX antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
NFIX
Additional Names:
NFIX, NF1A, MRSHSS, SOTOS2
Protein Accession Number:
NP_002492
Protein Gi Number:
56549649
Ncbi Gene Id Number:
4784
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Nuclear factor I (NFI) proteins constitute a family of sequence-specific transcription factors whose functional diversity is generated through transcription from four different genes (NFI-A, NFI-B, NFI-C, and NFI-X), alternative RNA splicing, and protein heterodimerization. NFI-X has divergent functions after binding in promoter or enhancer position. This property, combined with the differential expression of NFI-X, can achieve cell-type specificity of NFI dependent promoters and enhancers.
Background Reference 1:
Norquay, L.D., et al., (2003) Mol. Endocrinol. 17 (6), 1027-1038.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-679

List Size:
100 ul

List Price:

$519.00

Datasheet
Shipping Info

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