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Western blot analysis of lysed extracts from HL60 cells untreated or treated with TNF-α using NFκB-p105/p50 (Phospho-Ser927).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFκB-p105/p50 (Phospho-Ser927).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using NFκB-p105/p50 (Phospho-Ser927).
Western blot analysis of lysed extracts from HL60 cells untreated or treated with TNF-α using NFκB-p105/p50 (Phospho-Ser927).

NFkB p105 / p50 (phospho Ser927) Antibody

CATALOG NUMBER: 79-234

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
NFkB-p105/p50 (Phospho-Ser927) antibody was raised against a peptide sequence around phosphorylation site of serine 927 (C-D-S (p) -G-V) derived from Human NFκB-p105.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of NFκB-p105/p50 only when phosphorylated at serine 927.
Predicted Molecular Weight:
50, 120 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
NFKB1
Additional Names:
p50, KBF1, p105, EBP-1, NF-kB1, NFKB-p50, NFkappaB, NF-kappaB, NFKB-p105, NF-kappa-B, SHC, SHCA, DNA-binding factor KBF1, NF-kappa-B1 p84/NF-kappa-B1 p98, NFKB1, NFkB-p50
Protein Accession Number:
NP_001158884.1
Protein Gi Number:
259155302
Ncbi Gene Id Number:
4790
Background:
NF-κ-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-κ-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively.
Background Reference 1:
Hou S, et al. (2003) J Biol Chem. 278 (46): 45994-45998. Baeuerle P A, et al. (1994) Annu Rev Immunol. 12:141-179. Baeuerle P A, et al. (1996) Cell 87:13-20. Haskill S, et al. (1991) Cell 65:1281-1289.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-234

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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