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Western Blot at 1:1000 dilution Lane 1: Raji whole cell lysate Lane 2: A431 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:1000 dilution Lane 1: Raji whole cell lysate Lane 2: A431 whole cell lysate Lysates/proteins at 20 ug per lane.

NFKB1 Antibody

CATALOG NUMBER: 60-727

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Chicken, Mouse
Immunogen:
This NFKB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 911-939 amino acids from human NFKB1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
105 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NFKB1
Additional Names:
Nuclear factor NF-kappa-B p105 subunit, DNA-binding factor KBF1, EBP-1, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1, Nuclear factor NF-kappa-B p50 subunit, NFKB1
Protein Accession Number:
P19838
Protein Gi Number:
21542418
Ncbi Gene Id Number:
4790
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NFKB1 is a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth.
Background Reference 1:
Legembre, P., et al., J. Biol. Chem. 279(45):46742-46747 (2004).
Background Reference 2:
Imamura, R., et al., J. Biol. Chem. 279(45):46415-46423 (2004).
Background Reference 3:
Hung, J.H., et al., J. Biol. Chem. 279(45):46384-46392 (2004).
Background Reference 4:
Binnicker, M.J., et al., Infect. Immun. 72(11):6408-6417 (2004).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-727

List Size:
400 ul

List Price:

$495.00

Datasheet
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