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Antibody used in WB on Human Lung at 0.2-1 ug/ml.
Antibody used in WB on Human Lung at 0.2-1 ug/ml.

NFKBIA Antibody

CATALOG NUMBER: 31-231

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NFKBIA.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
NFKBIA antibody can be used for detection of NFKBIA by ELISA at 1:1562500. NFKBIA antibody can be used for detection of NFKBIA by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. XBL-10410 - Fetal Lung Tissue Lysate
Predicted Molecular Weight:
36 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store NFKBIA antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
NFKBIA
Additional Names:
NFKBIA, IKBA, MAD-3, NFKBI
Protein Accession Number:
NP_065390
Protein Gi Number:
10092619
Ncbi Gene Id Number:
4792
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).
Background Reference 1:
Janssen, R., et al., (2004) J. Exp. Med. 200 (5), 559-568.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

31-231

List Size:
100 ul

List Price:

$519.00

Datasheet
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