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Western blot analysis of extracts of various cell lines, using NFKBIB antibody (19-852) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat lung using NFKBIB antibody (19-852) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using NFKBIB antibody (19-852) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using NFKBIB antibody (19-852) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using NFKBIB antibody (19-852). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using NFKBIB antibody (19-852) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 30s.

NFKBIB Antibody

CATALOG NUMBER: 19-852

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-356 of human NFKBIB (NP_002494.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
MCF7
Positive Control 2:
293T
Positive Control 3:
Mouse liver
Positive Control 4:
Mouse skeletal muscle
Predicted Molecular Weight:
Observed: 40-50kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
NFKBIB
Additional Names:
NFKBIB, IKBB, TRIP9
Ncbi Gene Id Number:
4793
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene belongs to the NF-kappa-B inhibitor family, which inhibit NF-kappa-B by complexing with, and trapping it in the cytoplasm. Phosphorylation of serine residues on these proteins by kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B, which translocates to the nucleus to function as a transcription factor. Alternatively spliced transcript variants have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-852

List Size:
100 uL

List Price:

$436.00

Datasheet
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