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Western blot analysis of extracts of various cell lines, using NFU1 antibody (22-695) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded mouse intestine using NFU1 Antibody (22-695) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using NFU1 Antibody (22-695) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using NFU1 antibody (22-695) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

NFU1 Antibody

CATALOG NUMBER: 22-695

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human NFU1 (NP_001002755.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
BT-474
Positive Control 2:
SW480
Positive Control 3:
THP-1
Positive Control 4:
U-937
Positive Control 5:
Mouse liver
Positive Control 6:
Mouse heart
Predicted Molecular Weight:
Observed: 25-28kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
NFU1
Additional Names:
CGI-33, HIRIP, HIRIP5, MMDS1, NIFUC, Nfu, NifU, NFU1 iron-sulfur cluster scaffold homolog, mitochondrial, HIRA-interacting protein 5, NifU-like C-terminal domain containing, iron-sulfur cluster scaffold protein
Ncbi Gene Id Number:
27247
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a protein that is localized to mitochondria and plays a critical role in iron-sulfur cluster biogenesis. The encoded protein assembles and transfers 4Fe-4S clusters to target apoproteins including succinate dehydrogenase and lipoic acid synthase. Mutations in this gene are a cause of multiple mitochondrial dysfunctions syndrome-1, and pseudogenes of this gene are located on the short arms of chromosomes 1 and 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-695

List Size:
100 uL

List Price:

$436.00

Datasheet
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