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Antibody used in IHC on Human Liver cell lysates.
Antibody used in WB on Human Jurkat cells at 5.0-8.0 ug/ml.
Antibody used in IHC on Human Liver cell lysates.

NFYA Antibody

CATALOG NUMBER: 27-320

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NFYA.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
NFYA antibody can be used for detection of NFYA by ELISA at 1:312500. NFYA antibody can be used for detection of NFYA by western blot at 5.0-8.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
37 kDa, 34 kDa
Purification:
Antibody is supplied as total IgG.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store NFYA antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
NFYA
Additional Names:
NFYA, HAP2, CBF-A, CBF-B, NF-YA
Protein Accession Number:
NP_002496
Protein Gi Number:
4505389
Ncbi Gene Id Number:
4800
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NFYA is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms.
Background Reference 1:
Zhu, Q.S., et al., (2004) J.Biol.Chem.279(29):29902-29910.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-320

List Size:
100 ul

List Price:

$473.00

Datasheet
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