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Western blot analysis in HepG2 cell line lysates (35ug/lane).
Western blot analysis in HepG2 cell line lysates (35ug/lane).

NFYC Antibody

CATALOG NUMBER: 58-221

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This NFYC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-129 amino acids from the N-terminal region of human NFYC.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
50 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NFYC
Additional Names:
Nuclear transcription factor Y subunit gamma, CAAT box DNA-binding protein subunit C, Nuclear transcription factor Y subunit C, NF-YC, Transactivator HSM-1/2, NFYC
Protein Accession Number:
Q13952
Protein Gi Number:
20137773
Ncbi Gene Id Number:
4802
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes one subunit of a trimeric complexforming a highly conserved transcription factor that binds withhigh specificity to CCAAT motifs in the promoters of a variety ofgenes. The encoded protein, subunit C, forms a tight dimer with theB subunit, a prerequisite for subunit A association. The resultingtrimer binds to DNA with high specificity and affinity. Subunits Band C each contain a histone-like motif. Multiple transcriptvariants encoding different isoforms have been found for this gene.
Background Reference 1:
Ichikawa, S., et al. J. Bone Miner. Res. 25(8):1821-1829(2010)
Background Reference 2:
Murai-Takeda, A., et al. J. Biol. Chem. 285(11):8084-8093(2010)
Background Reference 3:
Ceribelli, M., et al. J. Biol. Chem. 284(49):34189-34200(2009)
Background Reference 4:
Yamanaka, K., et al. Genomics 94(4):219-227(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-221

List Size:
400 ul

List Price:

$495.00

Datasheet
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