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42-927 (1ug/ml) staining of Rat Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-927 (1ug/ml) staining of Rat Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

NGFR Antibody

CATALOG NUMBER: 42-927

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Rat
Homology:
Expected Species Reactivity based on sequence homology: Human, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-PEGSDSTAPSTQEP
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 75kDa band observed in Rat Heart lysates (calculated MW of 45.2kDa according to Human NP_002498.1 and 45.4kDa according to Rat NP_036742.2. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Nykjaer et al, Curr Opin Neurobiol. 2005 Feb;15(1):49-57). Recommended concentration: 1-3ug/ml.
Specificity:
This antibody is expected to recognize the extracellular domain of p75NTR.
Positive Control 1:
Cat. No. 1461 - Rat Heart Tissue Lysate
Predicted Molecular Weight:
Approx 75 kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
NGFR
Additional Names:
NGFR, nerve growth factor receptor, CD271, Gp80-LNGFR, TNFRSF16, p75(NTR), p75NTR, NGF receptor, OTTHUMP00000220319, TNFR superfamily, member 16, low affinity nerve growth factor receptor, low affinity neurotrophin receptor p75NTR, low-affinity nerve growth factor receptor, p75 ICD, tumor necrosis factor receptor superfamily member 16
Protein Accession Number:
NP_002498.1
Protein Gi Number:
4505393
Ncbi Gene Id Number:
4804
User Note:
The observed molecular weight corresponds to earlier findings in literature with different antibodies (Nykjaer et al, Curr Opin Neurobiol. 2005 Feb;15 (1) :49-57).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-927

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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