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Western blot analysis of extracts of HT-1080 cells, using NGFR antibody (13-894).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded mouse uterus using NGFR antibody (13-894) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human uterus using NGFR antibody (13-894) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of N2A cells using NGFR antibody (13-894).
Immunofluorescence analysis of HeLa cells using NGFR antibody (13-894).
Western blot analysis of extracts of HT-1080 cells, using NGFR antibody (13-894).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

NGFR Antibody [B1E5]

CATALOG NUMBER: 13-894

Clonality:
Monoclonal
Tested Applications:
IF, IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein of human NGFR
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, IP, WB
Application Note:
WB: 1:10000 - 1:20000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:20 - 1:50
Positive Control 1:
HT-1080
Predicted Molecular Weight:
Observed: 70kDa
Purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
NGFR
Additional Names:
NGFR Antibody: CD271, p75NTR, TNFRSF16, p75(NTR), Gp80-LNGFR, Tumor necrosis factor receptor superfamily member 16, NGF receptor
Ncbi Gene Id Number:
4804
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-894

List Size:
50 uL

List Price:

$335.00

Out of stock

Datasheet
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