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45-967 (1ug/ml) staining of Daudi lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-967 (1ug/ml) staining of Daudi lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

NHERF2 Antibody

CATALOG NUMBER: 45-967

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-DLPGSDKDTEESGLH
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 35kDa band observed in lysates of cell lines HEK293 and Daudi (calculated MW of 36.2kDa according to NP_004776.3). Recommended concentration: 1-3ug/ml.
Specificity:
This antibody is expected to recognize isoforms b and e (NP_004776.3 and NP_001239005.1) only.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SLC9A3R2
Additional Names:
SLC9A3R2, solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 2, HGNC:11076, E3KARP, NHE3RF2, NHERF-2, NHERF2, OCTS2, SIP-1, SIP1, TKA-1, sodium/hydrogen exchanger, solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 2, solute carrier family 9 isoform 3 regulator 2, solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 2, MGC104639, solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 2
Protein Accession Number:
NP_004776.3,NP_001239005.1
Protein Gi Number:
71897229
Ncbi Gene Id Number:
9351
Background Reference 1:
Cha B, Kim JH, Hut H, Hogema BM, Nadarja J, Zizak M, Cavet M, Lee-Kwon W, Lohmann SM, Smolenski A, Tse CM, Yun C, de Jonge HR, Donowitz M. cGMP inhibition of Na+/H+ antiporter 3 (NHE3) requires PDZ domain adapter NHERF2, a broad specificity protein kinase G-anchoring protein. J Biol Chem. 2005 Apr 29;280(17):16642-50. Epub 2005 Feb 18.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-967

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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