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Immunohistochemistry of NINJ1 in mouse brain tissue with NINJ1 antibody at 5 μg/ml.
Immunofluorescence of NINJ1 in mouse brain tissue with NINJ1 antibody at 20 μg/ml.
Flow Cytometry Validation of NINJ1 in HeLa Cells 1X10^6/mL HeLa cells were intracellularly stained with 2 μg/mL of NINJ1 antibody (red) or Rabbit IgG isotype control (black) at RT for 30 min, followed by PSFlo 488 conjugated goat anti-rabbit IgG secondary antibody staining (1:1000) at RT for 30 min. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100 in PBS.
Immunohistochemistry of NINJ1 in mouse brain tissue with NINJ1 antibody at 5 μg/ml.
Trial Size

NINJ1 Antibody

CATALOG NUMBER: 7967

Clonality:
Polyclonal
Tested Applications:
ELISA, FC, IHC-P, IHC-P/IF
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
NINJ1 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human NINJ1.
The immunogen is located within the last 50 amino acids of NINJ1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, FC, IHC-P, IHC-P/IF
Application Note:
IHC-P/IF: 20 μg/mL; IHC-P: 5 μg/mL; FC: 2 μg/mL.
Antibody validated: Immunohistochemistry and Immunofluorescence in mouse samples; Flow Cytometry in human samples. All other applications and species not yet tested.
Specificity:
NINJ1 antibody is human, mouse and rat reactive.
Predicted Molecular Weight:
Predicted: 17 kDa
Observed: 16 kDa
Purification:
NINJ1 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
NINJ1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
NINJ1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
NINJ1
Additional Names:
NINJ1 Antibody: NIN1, NINJURIN, Ninjurin-1, Nerve injury-induced protein 1
Protein Accession Number:
NP_004139
Protein Gi Number:
148922911
Ncbi Gene Id Number:
4814
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Ninjurin-1 (NINJ1) is a homophilic adhesion molecule that is strongly induced in dorsal root ganglion (DRG) and Schwann cells following nerve injury and promotes neurite outgrowth of DRG neurons (1,2), suggesting that NINJ1 may play a role in nerve regeneration. NINJ1 is transcriptionally regulated by p53 and is induced by DNA damage in a p53-dependent manner. A deficiency of NINJ1 increases p53 expression, suppresses cell proliferation, but enhances apoptosis and premature senescence in a p53-dependent manner as well as radiation-induced cell mortality (3).
Background Reference 1:
Araki T and Milbrandt J. Ninjurin, a novel adhesion molecule, is induced by nerve injury and promotes axonal growth. Neuron 1996; 17:353-61.
Background Reference 2:
Araki T, Zimonjic DB, Popescu NC, et al. Mechanism of homophilic binding mediated by ninjurin, a novel widely expressed adhesion molecule. J. Biol. Chem. 1997; 212:21373-80.
Background Reference 3:
Cho SJ, Rossi A, Jung YS, et al. Ninjurin1, a target of p53, regulates p53 expression and p53-dependent cell survival, sensecence, and radiation-induced mortality. Proc. Natl. Acad. Sci. USA 2013; 110:9362-7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7967

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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