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Western blot analysis of NIPSNAP3B in mouse brain tissue lysate with NIPSNAP3B antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of NIPSNAP3B in mouse brain tissue with NIPSNAP3B antibody at 2.5 μg/mL.
Immunofluorescence of NIPSNAP3B in mouse brain tissue with NIPSNAP3B antibody at 5 μg/mL.
Western blot analysis of NIPSNAP3B in mouse brain tissue lysate with NIPSNAP3B antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

NIPSNAP3B Antibody

CATALOG NUMBER: 4957

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
NIPSNAP3B antibody was raised against a 16 amino acid synthetic peptide near the center of human NIPSNAP3B.
The immunogen is located within amino acids 40 - 90 of NIPSNAP3B.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
NIPSNAP3B antibody can be used for detection of NIPSNAP3B by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
NIPSNAP3B antibody is predicted to not cross-react with any other members of the NIPSNAP protein family.
Positive Control 1:
Cat. No. 1403 - Mouse Brain Tissue Lysate
Purification:
NIPSNAP3B Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
NIPSNAP3B Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
NIPSNAP3B antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NIPSNAP3B
Additional Names:
NIPSNAP3B Antibody: NIPSNAP3, NIPSNAP3, Protein NipSnap homolog 3B, SNAP1, NipSnap3B
Protein Accession Number:
Q9BS92
Protein Gi Number:
17380145
Ncbi Gene Id Number:
55335
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NIPSNAP3B Antibody: The NIPSNAP proteins comprise a family of evolutionarily well-conserved proteins with strong sequence similarity to the central portion of a protein encoded by C. elegans chromosome III between a 4-nitrophenylphosphatase (NIP) domain and non-neuronal SNAP25-like protein. NIPSNAP2, a novel gene encoding a protein with tyrosine phosphorylation sites and a transmembrane domain, is co-amplified with EGFR in approximately 40% of glioblastomas, the most common and malignant form of central nervous system tumors. While NIPSNAP3B is highly expressed skeletal muscle, NIPSNAP3A mRNA levels are low. NIPSNAP proteins have been suggested to be important in vesicular transport.
Background Reference 1:
Seroussi E, Pan HQ, Kedra D, et al. Characterization of the human NIPSNAP1 gene from 22q12: a member of a novel gene family. Gene1998; 212:13-20.
Background Reference 2:
Wang X-Y, Smith DI, Liu W, et al. GBAS, a novel gene encoding a protein with tyrosine phosphorylation sites and a transmembrane domain, is co-amplified with EGFR. Genomics1998; 49:448-51.
Background Reference 3:
Buechler C, Bodzioch M, Bared SM, et al. Expression pattern and raft association of NIPSNAP3 and NIPSNAP4, highly homologous proteins encoded by genes in close proximity to the ATP-binding cassette transporter A1. Genomics2004; 83:1116-24.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4957

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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