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Western blot analysis of NK3R in RAW264.7 cell lysate with NK3R antibody at (A) 0.5 and (B) 1 μg/mL.
Immunohistochemistry of NK3R in human brain tissue with NK3R antibody at 5 μg/mL.
Immunofluorescence of NK3R in Human Brain tissue with NK3R antibody at 20 ug/mL.
Western blot analysis of NK3R in RAW264.7 cell lysate with NK3R antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

NK3R Antibody

CATALOG NUMBER: 4255

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (94%), Rabbit: (94%), Guinea pig: (83%)
Immunogen:
NK3R antibody was raised against a 18 amino acid synthetic peptide from near the center of human NK3R.
The immunogen is located within amino acids 370 - 420 of NK3R.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
NK3R antibody can be used for detection of NK3R by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
NK3R antibody does not recognize NK1R or NK2R.
Positive Control 1:
Cat. No. 1207 - Raji Cell Lysate
Positive Control 2:
Cat. No. 1303 - Human Brain Tissue Lysate
Positive Control 3:
Cat. No. 10-301 - Human Brain Tissue Slide
Predicted Molecular Weight:
Predicted: 51 kDa
Observed: 51 kDa
Purification:
NK3R Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
NK3R Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
NK3R antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TACR3
Additional Names:
NK3R Antibody: NKR, HH11, NK3R, NK-3R, TAC3RL, TAC3R, Neuromedin-K receptor, NK-3 receptor, NKR
Protein Accession Number:
P29371
Protein Gi Number:
128364
Ncbi Gene Id Number:
6870
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NK3R Antibody: The tachykinins are a family of small peptides that include the neurotransmitters substance P, neurokinin A, and neurokinin B, which can act on three related but distinct seven transmembrane G-proteins coupled receptors, albeit at different concentrations. The NK-3 receptor (NK3R) has greatest affinity for neurokinin B and is highly expressed in the supraoptic and paraventricular nuclei. Following binding of its ligand, NK3R activates a phosphatidylinositol-calcium second messenger system. It is likely these signals lead to the release of vasopressin and oxytocin into the circulation. NK3R may be involved in learning and memory as mice lacking this gene expressed cognitive deficits compared to normal mice. Although it has been suggested that NK3R plays a role in the regulation of vagal afferent relay neurons, it is likely that these receptors are activated by substance P or neurokinin A, as the airway nerves do not express neurokinin B.
Background Reference 1:
Maggi CA. The mammalian tachykinin receptors. Gen. Pharmacol. 1995; 26:911-44.
Background Reference 2:
Ding Y-Q, Shi J, Su L-Y, et al. Receptor (NK3)-containing neurons in the paraventricular and supraoptic nuclei of the rat hypothalamus synthesize vasopressin and express fos following intravenous injection of hypotonic saline. Neurosci. 1999; 91:1077-85.
Background Reference 3:
Nakajima Y, Tsuchida K, Negishi M, et al. Direct linkage of three tachykinin receptors to stimulation of both phosphatidylinositol hydrolysis and cyclic AMP cascades in transfected Chinese hamster ovary cells. J. Biol. Chem. 1992; 267:2437-42.
Background Reference 4:
Haley GE and Flynn FW. Tachykinin NK3 receptor contribution to systemic release of vasopressin and oxytocin in response to osmotic and hypotensive challenge. Am. J. Regul. Integr. Comp. Physiol. 2007; 293:R931-7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4255

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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