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Western blot analysis in Jurkat cell line lysates (35ug/lane).
NKD2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of NKD2 Antibody with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in Jurkat cell line lysates (35ug/lane).

NKD2 Antibody

CATALOG NUMBER: 56-250

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This NKD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366-393 amino acids from the C-terminal region of human NKD2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
50 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NKD2
Additional Names:
Protein naked cuticle homolog 2, Naked-2, hNkd2, NKD2
Protein Accession Number:
Q969F2
Protein Gi Number:
74716653
Ncbi Gene Id Number:
85409
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
In the mouse, Nkd is a Dishevelled (see DVL1; MIM601365)-binding protein that functions as a negative regulator ofthe Wnt (see WNT1; MIM 164820)-beta-catenin (see MIM 116806)-Tcf(see MIM 602272) signaling pathway.
Background Reference 1:
Hu, T., et al. J. Biol. Chem. 285(18):13561-13568(2010)
Background Reference 2:
Ding, W., et al. Proc. Natl. Acad. Sci. U.S.A. 105(36):13433-13438(2008)
Background Reference 3:
Li, C., et al. Mol. Biol. Cell 18(8):3081-3093(2007)
Background Reference 4:
Adamowicz, M., et al. Genes Chromosomes Cancer 45(9):829-838(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-250

List Size:
400 ul

List Price:

$495.00

Datasheet
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