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Western blot analysis of NKX2-1 expression in human lung tissue lysate with NKX2-1 antibody at (A) 0.25 and (B) 0.5 μg/ml.
Immunohistochemistry of NKX2-1 in human lung tissue with NKX2-1 antibody at 2.5 μg/mL.
Immunofluorescence of NKX2-1 in human lung tissue with NKX2-1 antibody at 20 μg/mL.
Western blot analysis of NKX2-1 expression in human lung tissue lysate with NKX2-1 antibody at (A) 0.25 and (B) 0.5 μg/ml.
Trial Size

NKX2-1 Antibody

CATALOG NUMBER: 6741

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
NKX2-1 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human NKX2-1.
The immunogen is located within the first 50 amino acids of NKX2-1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
NKX2-1 antibody can be used for detection of NKX2-1 by Western blot at 0.25 – 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
NKX2-1 antibody is predicted to not cross-react with other NK2 homeobox family members. At least three isoforms of NKX2-1 are known to exist; this antibody will detect all three.
Positive Control 1:
Cat. No. 1302 - Human Lung Tissue Lysate
Positive Control 2:
Cat. No. 10-101 - Human Lung Tissue Slide
Predicted Molecular Weight:
Predicted: 46 kDa
Observed: 41, 45 kDa
Purification:
NKX2-1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
NKX2-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
NKX2-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NKX2-1
Additional Names:
NKX2-1 Antibody: BCH, BHC, NK-2, TEBP, TTF1, NKX2A, TITF1, TTF-1, NKX2.1, Homeobox protein NK-2 homolog A
Protein Accession Number:
P43699
Protein Gi Number:
118766339
Ncbi Gene Id Number:
7080
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NKX2-1 Antibody: NKX2-1 (NK2 homeobox 1) has been identified as a thyroid-specific transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin and thyrotropin receptor. NKX2-1 is crucial in the maintenance of the thyroid differentiation phenotype and morphogenesis. It contains one homeobox DNA-binding domain. NKX2-1 also may play a role in lung development and surfactant homeostasis. Mutations and deletions in this gene are associated with benign hereditary chorea, congenital hypothyroidism, neonatal respiratory distress, and may be associated with thyroid cancer. NKX2-1 has been suggested to be a candidate suppressor of malignant progression.
Background Reference 1:
Lazzaro D, Price M, Felice MD, et al. The transcription factor TTF-1 is expressed at the onset of thyroid and lung morphogenesis and in restricted regions of the fetal brain. Development 1991; 113:1093-1104.
Background Reference 2:
Guazzi S, Price M, De Felice M, et al. Thyroid nuclear factor 1 (TTF-1) contains a homeodomain and displays a novel DNA binding specificity. EMBO J. 1990; 9:3631-9.
Background Reference 3:
Breedveld GJ, Van Dongen JWF et al. Mutations in TITF-1 are associated with benign hereditary chorea. Hum. Mol. Genet. 2002; 11:9719.
Background Reference 4:
Kondo T, Nakazawa T, Ma D, et al. Epigenetic silencing of TTF-1/NKX2-1 through DNA hypermethylation and histone H3 modulation in thyroid carcinomas. Lab Invest. 2009; 89:791-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6741

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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