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Antibody used in WB on Mouse SP2/0 at 0.125 ug/ml.
Antibody used in WB on Mouse SP2/0 at 0.125 ug/ml.

NKX2-2 Antibody

CATALOG NUMBER: 28-618

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse NKX2-2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
NKX2-2 antibody can be used for detection of NKX2-2 by ELISA at 1:1562500. NKX2-2 antibody can be used for detection of NKX2-2 by western blot at 0.125 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
SP2/0 Cell Lysate
Predicted Molecular Weight:
30 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store NKX2-2 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
NKX2-2
Additional Names:
NKX2-2, Nkx2.2, tinman, Nkx-2.2
Protein Accession Number:
NP_035049
Protein Gi Number:
6754860
Ncbi Gene Id Number:
18088
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Nkx2-2 contains 1 homeobox DNA-binding domain which is essential for interaction with OLIG2. Nkx2-2 may be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance.
Background Reference 1:
Sun, T., et al., (2006) Dev. Biol. 292 (1), 152-164.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-618

List Size:
100 ul

List Price:

$519.00

Datasheet
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