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Western blot analysis of NKX2-2 in rat kidney tissue lysate with NKX2-2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of NKX2-2 in human kidney tissue with NKX2-2 antibody at 2.5 μg/mL.
Immunofluorescence of NKX2-2 in human kidney tissue with NKX2-2 antibody at 20 μg/mL.
Western blot analysis of NKX2-2 in rat kidney tissue lysate with NKX2-2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

NKX2-2 Antibody

CATALOG NUMBER: 6743

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
NKX2-2 antibody was raised against a 19 amino acid synthetic peptide near the center of human NKX2-2.
The immunogen is located within amino acids 90 - 140 of NKX2-2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
NKX2-2 antibody can be used for detection of NKX2-2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
NKX2-2 antibody is predicted to not cross-react with other NK2 homeobox family members.
Positive Control 1:
Cat. No. 1465 - Rat Kidney Tissue Lysate
Positive Control 2:
Cat. No. 10-401 - Human Kidney Tissue Slide
Purification:
NKX2-2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
NKX2-2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
NKX2-2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NKX2-2
Additional Names:
NKX2-2 Antibody: NKX2B, NKX2.2, NKX2B, Homeobox protein NK-2 homolog B
Protein Accession Number:
NP_002500
Protein Gi Number:
4505401
Ncbi Gene Id Number:
4821
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NKX2-2 Antibody: NKX2-2 (NK2 homeobox 2) is a member of a family of transcription factors that are involved in embryonic development and cell fate. NKX2-2 is crucial in the development of pancreatic islet cell differentiation and activates the ghrelin-promoter in these cells. NKX2-2 is expressed at the onset of pancreatic epithelium formation and becomes restricted to mature α-, β- and PP cells, and in NKX2-2-null mice embryos, all β-cells and most a-cells are replaced by ghrelin-positive cells. NKX2-2 is also thought to be involved in ventral neuronal patterning and floor plate development and commissural axon guidance.
Background Reference 1:
Sussel L, Kalamaras J, Hartigan-O'Connor DJ, et al. Mice lacking the homeodomain transcription factor Nkx2.2 have diabetes due to arrested differentiation of pancreatic beta cells. Development 1998; 125:2213-21
Background Reference 2:
Prado CL, Pugh-Bernard AE, Elghazi L, et al. Ghrelin cells replace insulin-producing beta cells in two mouse models of pancreas development. Proc. Natl. Acad. Sci. 2004; 101:2924-9.
Background Reference 3:
Hill JT, Chao CS, Anderson KR, et al. Nkx2.2 activates the ghrelin promoter in pancreatic islet cells. Mol. Endocrinol. 2010; 381-90.
Background Reference 4:
Briscoe J, Sussel L, Serup P, et al. Homeobox gene Nkx2.2 and specification of neuronal identity by graded Sonic hedgehog signaling. Nature 1999; 398:622-7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6743

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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