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Western blot analysis of NKX2-4 in A20 cell lysate with NKX2-4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry of NKX2-4 in A20 cells with NKX2-4 antibody at 2.5 μg/mL.
Immunofluorescence of NKX2-4 in A20 cells with NKX2-4 antibody at 20 μg/mL.
Western blot analysis of NKX2-4 in A20 cell lysate with NKX2-4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

NKX2-4 Antibody

CATALOG NUMBER: 6747

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Guinea pig: (100%), Rat: (86%), Chicken: (86%), Bovine: (71%)
Immunogen:
NKX2-4 antibody was raised against an 18 amino acid synthetic peptide near the center of human NKX2-4.
The immunogen is located within amino acids 220 - 270 of NKX2-4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
NKX2-4 antibody can be used for detection of NKX2-4 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
NKX2-4 antibody is predicted to not cross-react with other NK2 homeobox family members. At least two isoforms of NKX2-4 are known to exist; this antibody will detect both isoforms.
Positive Control 1:
Cat. No. 1288 - A20 Cell Lysate
Positive Control 2:
Cat. No. 17-208 - A-20 Cell Slide
Purification:
NKX2-4 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
NKX2-4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
NKX2-4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NKX2-4
Additional Names:
NKX2-4 Antibody: NKX2D, NKX2.4, NKX2D, Homeobox protein NK-2 homolog D
Protein Accession Number:
NP_149416
Protein Gi Number:
157426823
Ncbi Gene Id Number:
644524
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NKX2-4 Antibody: NKX2-4 (NK2 homeobox 4) is a member of a family of transcription factors that are involved in embryonic development and cell fate. NKX2-4 is closely related to NKX2-1 and NKX2-4 and is specifically localized to the ventral diencephalon. Recent experiments in Xenopus have suggested that the homeodomain protein XANF is necessary for the activation of NKX2-4, but does not directly regulate its expression.
Background Reference 1:
Wang CC, Brodnicki T, Copeland NG, et al. Conserved linkage of NK-2 homeobox gene pairs Nkx2-2/2-4 and Nkx2-1/2-9 in mammals. Mamm. Genome 2000; 11:466-8.
Background Reference 2:
Small EM, Vokes SA, Garriock RJ, et al. Developmental expression of the Xenopus Nkx2-1 and Nkx2-4 genes. Mech. Dev. 2000; 96:259-62.
Background Reference 3:
Hill JT, Chao CS, Anderson KR, et al. Nkx2.2 activates the ghrelin promoter in pancreatic islet cells. Mol. Endocrinol. 2010; 381-90.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6747

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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