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Western blot analysis of NKX2-6 in mouse heart tissue lysate with NKX2-6 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of NKX2-6 in human spleen tissue with NKX2-6 antibody at 5 μg/mL.
Immunofluorescence of NKX-6 in human spleen tissue with NKX2-6 antibody at 20 μg/mL.
Western blot analysis of NKX2-6 in mouse heart tissue lysate with NKX2-6 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

NKX2-6 Antibody

CATALOG NUMBER: 6751

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
NKX2-6 antibody was raised against a 15 amino acid synthetic peptide near the center of human NKX2-6.
The immunogen is located within amino acids 170 - 220 of NKX2-6.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
NKX2-6 antibody can be used for detection of NKX2-6 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
NKX2-6 antibody is predicted to not cross-react with other NK2 homeobox family members. At least two isoforms of NKX2-6 are known to exist; this antibody will detect both isoforms
Positive Control 1:
Cat. No. 1401 - Mouse Heart Tissue Lysate
Positive Control 2:
Cat. No. 10-901 - Human Spleen Tissue Slide
Purification:
NKX2-6 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
NKX2-6 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
NKX2-6 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NKX2-6
Additional Names:
NKX2-6 Antibody: CSX2, NKX2F, NKX4-2, Homeobox protein NK-2 homolog F
Protein Accession Number:
NP_001129743
Protein Gi Number:
343183350
Ncbi Gene Id Number:
137814
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NKX2-6 Antibody: NKX2-6 (NK2 homeobox 6) is a member of a family of transcription factors that are involved in embryonic development and cell fate. NKX2-6 is a vertebrate homolog of Drosophila homeobox-containing protein called 'tinman', which has been shown to be essential for development of the heart-like dorsal vessel. In conjunction with related gene, NKX2-5, this gene may play a role in both pharyngeal and cardiac embryonic development. Mutations in this gene are thought to be a cause for some congenital heart abnormalities.
Background Reference 1:
Copeland NG, Jenkins NA, and Harvey RP. The murine homeobox genes Nkx2.3 and Nkx2.6 are located on chromosomes 19 and 14, respectively. Genomics 1994; 22:655-6.
Background Reference 2:
Tanaka M, Kasahara H, Bartunkova S, et al. Vertebrate homologs of tinman and bagpipe: roles of the homeobox genes in cardiovascular development. Dev. Genet. 1998; 22:239-49.
Background Reference 3:
Biben C, Hatzistavrou T, and Harvey RP. Expression of NK-2 class homeobox gene Nkx2-6 in foregut endoderm and heart. Mech. Dev. 1998; 73:125-7
Background Reference 4:
Tanaka M, Schinke M, Liao HS, et al. Nkx2.5 and Nkx2.6, homologs of Drosophila tinman, are required for development of the pharynx. Mol. Cell. Biol. 2001; 21:4391-8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6751

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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