Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of NKX2-8 in rat liver tissue lysate with NKX2-8 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide
Immunohistochemistry of NKX2-8 in human liver tissue with NKX2-8 antibody at 2.5 μg/mL.
Immunofluorescence of NKX-8 in human liver tissue with NKX2-8 antibody at 20 μg/mL.
Western blot analysis of NKX2-8 in rat liver tissue lysate with NKX2-8 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide
Trial Size

NKX2-8 Antibody

CATALOG NUMBER: 6753

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
NKX2-8 antibody was raised against a 19 amino acid synthetic peptide near the center of human NKX2-8.
The immunogen is located within amino acids 60 - 110 of NKX2-8.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
NKX2-8 antibody can be used for detection of NKX2-8 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
NKX2-8 antibody is predicted to not cross-react with other NK2 homeobox family members.
Positive Control 1:
Cat. No. 1464 - Rat Liver Tissue Lysate
Positive Control 2:
Cat. No. 10-201 - Human Liver Tissue Slide
Purification:
NKX2-8 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
NKX2-8 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
NKX2-8 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NKX2-8
Additional Names:
NKX2-8 Antibody: NKX2H, NKX2.8, Nkx2-9, NKX-2.8, NKX2G, NKX2H, Homeobox protein NK-2 homolog H
Protein Accession Number:
NP_055175
Protein Gi Number:
31377777
Ncbi Gene Id Number:
26257
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NKX2-8 Antibody: NKX2-8 (NK2 homeobox 8) is a member of a family of transcription factors that are involved in embryonic development and cell fate. It is expressed in the ventral foregut, the developing heart, the epithelial layers of the branchial arches and in the dorsal mesoderm. In conjunction with related protein, NKX2-5, NKX2-8 may play a role in cardiac embryonic development. NKX2-8 is also thought to be involved in lung development and is suspected of being an oncogene in lung cancer that is activated by way of gene amplification at chromosome 14q13.
Background Reference 1:
Boettger T, Stein S, and Kessel M. The chicken NKX2.8 homeobox gene: A novel member of the NK-2 gene family. Dev. Genes Evol. 1997; 207:65-70.
Background Reference 2:
Kendall J, Liu Q, Bakleh A, et al. Oncogenic ccooperation and coamplification of developmental transcription factor genes in lung cancer. Proc. Natl. Acad. Sci. USA 2007; 104:16663-8.
Background Reference 3:
Hsu DS, Acharya CR, Balakumaran BS, et al. Characterizing the developmental pathways TTF-1, NKX2-8, and PAX9 in lung cancer. Proc. Natl. Acad. Sci. USA 2009; 106:5312-7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6753

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369