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Western blot analysis of extracts of various cell lines, using NLGN4X antibody (23-330) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Western blot analysis of extracts of various cell lines, using NLGN4X antibody (23-330) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

NLGN4X Antibody

CATALOG NUMBER: 23-330

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 697-816 of human NLGN4X (NP_065793.1).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:500 - 1:2000
Positive Control 1:
BT-474
Positive Control 2:
Mouse brain
Positive Control 3:
Mouse thymus
Positive Control 4:
Rat brain
Predicted Molecular Weight:
Observed: 92kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
NLGN4X
Additional Names:
ASPGX2, AUTSX2, HLNX, HNL4X, HNLX, KIAA1260, MGC22376, neuroligin 4, X-linked, neuroligin X, neuroligin-4, NLGN, NLGN4, NLGN4X, OTTHUMP00000022863, OTTHUMP00000022864, OTTHUMP00000022865 , UNQ365/PRO701
Ncbi Gene Id Number:
57502
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the type-B carboxylesterase/lipase protein family. The encoded protein belongs to a family of neuronal cell surface proteins. Members of this family may act as splice site-specific ligands for beta-neurexins and may be involved in the formation and remodeling of central nervous system synapses. The encoded protein interacts with discs large homolog 4 (DLG4). Mutations in this gene have been associated with autism and Asperger syndrome. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-330

List Size:
100 uL

List Price:

$419.00

Out of stock

Datasheet
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