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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human NLRP2 with DYKDDDDK tag probed with 42-532 (1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/5000) in lane C. Mock-transfected HEK293 probed with 42-532 (1mg/ml) in Lane B. Primary
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human NLRP2 with DYKDDDDK tag probed with 42-532 (1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/5000) in lane C. Mock-transfected HEK293 probed with 42-532 (1mg/ml) in Lane B. Primary

NLRP2 Antibody

CATALOG NUMBER: 42-532

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-KNPQLIIDTEKHHP
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:In transfected HEK293 transiently expressing full-length Human NLRP2 (myc and DYKDDDDK tagged), a band of approx. 150kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 1-2ug/ml.Purchase the +ve control! The transfected lysate used in QC is available for sale. Please email sales@everestiotech.com to request datasheet, pricing and delivery information.
Positive Control 1:
Transfected HEK293
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
NLRP2
Additional Names:
NLRP2, NLR family, pyrin domain containing 2, CLR19.9, FLJ20510, NALP2, NBS1, PAN1, PYPAF2, NACHT, LRR and PYD containing protein 2, NACHT, leucine rich repeat and PYD containing 2, PYRIN-Containing APAF1-like, nucleotide-binding oligomerization domain, l
Protein Accession Number:
NP_060322.1
Protein Gi Number:
8923473
Ncbi Gene Id Number:
55655
Background Reference 1:
Ji S, Shin JE, Kim YS, Oh JE, Min BM, Choi Y, Toll-like receptor 2 and NALP2 mediate induction of human beta-defensins by fusobacterium nucleatum in gingival epithelial cells. Infection and immunity 2009 Mar 77 (3): 1044-52.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-532

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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