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Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
NMB Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of NMB Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).

NMB Antibody

CATALOG NUMBER: 61-894

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This NMB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-42 amino acids from the Central region of human NMB.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
13 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NMB
Additional Names:
Neuromedin-B, Neuromedin-B-32, Neuromedin-B, NMB
Protein Accession Number:
P08949
Protein Gi Number:
281185514
Ncbi Gene Id Number:
4828
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NMB stimulates smooth muscle contraction in a manner similar to that of bombesin.
Background Reference 1:
Luttrell, L.M. Mol. Biotechnol. 39(3):239-264(2008)
Background Reference 2:
Spalova, J., et al. Physiol Res 57 SUPPL 1, S39-S48 (2008)
Background Reference 3:
Hoggard, N., et al. J. Mol. Endocrinol. 39(3):199-210(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-894

List Size:
400 ul

List Price:

$495.00

Datasheet
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