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Western blot analysis of anti-NMD3 Pab in mouse heart (left) and T47D (right)tissue lysates (35ug/lane)
Western blot analysis of anti-NMD3 Pab in mouse heart (left) and T47D (right)tissue lysates (35ug/lane)

NMD3 Antibody

CATALOG NUMBER: 60-570

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This NMD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 410-440 amino acids from the C-terminal region of human NMD3.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
58 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NMD3
Additional Names:
60S ribosomal export protein NMD3, hNMD3, NMD3
Protein Accession Number:
Q96D46
Protein Gi Number:
74731412
Ncbi Gene Id Number:
51068
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
It has been suggested that NMD3 is a cytoplasmic factor required for a late cytoplasmic assembly step of the 60S subunit but is not a ribosomal protein. A mutation in NMD3 was found to be lethal in the absence of XRN1, which encodes the major cytoplasmic exoribonuclease responsible for mRNA turnover. The NMD3 protein sequence does not contain readily recognizable motifs of known function. However, NMD3 orthologues display an amino-terminal domain containing four repeats of Cx2C, reminiscent of zinc-binding proteins, implicated in nucleic acid binding or protein oligomerization.
Background Reference 1:
Trotta, C.R., et al., EMBO J. 22(11):2841-2851 (2003).
Background Reference 2:
Ho, J.H. et al., Mol. Cell. Bio., 19(3):2389-2399 (1999).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-570

List Size:
400 ul

List Price:

$495.00

Datasheet
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