Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

NMDA NR2A Subunit Antibody
NMDA NR2A Subunit Antibody

NMDA NR2A Antibody

CATALOG NUMBER: XPS-2024

Clonality:
Polyclonal
Tested Applications:
IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Immunogen:
NMDA NR2A polyclonal antibody was raised against a C-terminal fusion protein of the NR2A subunit of the NMDAR.
Conjugate:
Unconjugated
Tested Applications:
IHC, IP, WB
Application Note:
NMDA NR2A Subunit antibody is specific for the 180k NR2A subunit in Western blots of most brain regions. Immunolabeling blocked by pre-adsorption of antibody with the C-terminal fusion protein of the NR2A subunit that was used to generate the antibody. No reactivity towards the NR2B subunit of the NMDAR. Applications include Dot Blots (DB), Immunohistochemistry (IHC), Immunoprecipitation (IP) and Western Blots (WB). Human, mouse, and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for IHC, DB and WB, and 3 μg/200 μg lysate will quantitatively precipitate all NMDAR 2A for IP.
Predicted Molecular Weight:
180
Purification:
Affinity Purified
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
NMDA NR2A Subunit antibody can be stored at -20°C. After reconstitution in 50 μl PBS, the antibody should be aliquot and stored at -20°C and is stable at -20°C for at least 1 year. Ambient shipment is suitable for both international and domestic.
Ncbi Official Symbol:
Grin2a
Protein Accession Number:
Q00959
Protein Gi Number:
3915771
Ncbi Gene Id Number:
24409
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death. Increased membrane surface expression of the NR2A subunit of the receptor has been associated synaptic plasticity.
Background Reference 1:
Loftis, J.M. and Janowsky, A., "The N-methyl-D-aspartate receptor subunit NR2B: localization, functional properties, regulation, and clinical implications," Pharmacol. Ther., 97 (2003) 55 - 85.
Background Reference 2:
Clayton, D.A., Mesches, M.H., Alvarez, E., Bickford, P.C., and Browning, M.D., "A hippocampal NR2B deficit can mimic age-related changes in long-term potentiation and spatial learning in the Fischer 344 rat," J. Neurosci., 22 (2002) 3628 - 3637.
Background Reference 3:
Grosshans, D.R., Clayton, D.A., Coultrap, S.J., and Browning, M.D., "LTP leads to rapid surface expression on NMDA not AMPA receptors in adult rat CA1," Nature Neurosci., 5 (2002) 27 - 33.
Background Reference 4:
Snell, L.D., Nunley, K.R., Lickteig, R.L., Browning, M.D., Tabakoff, B., and Hoffman, P.L., "Regional and subunit specific changes in NMDA receptor mRNA and immunoreactivity in mouse brain following chronic ethanol ingestion," Mol. Brain Res., 40 (1996) 71 - 78.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XPS-2024

List Size:
10 ug

List Price:

$540.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369