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43-626 (0.01ug/ml) staining of A549 (A) and HeLa (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-626 (0.01ug/ml) staining of A549 (A) and HeLa (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

NME1 Antibody

CATALOG NUMBER: 43-626

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: DYTSCAQNWIYE
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 20+18kDa bands observed in lysates of cell lines A549, HeLa and HepG2 (calculated MW of 19.7kDa according to NP_937818.1 and 17.1kDa according to NP_000260.1). Recommended concentration: 0.01-0.03ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_937818.1; NP_000260.1).
Predicted Molecular Weight:
Approx 20 + 18kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
NME1
Additional Names:
NME1, AWD, GAAD, NB, NBS, NDPK-A, NDPKA, NM23, NM23-H1, non-metastatic cells 1, protein (NM23A) expressed in, NDP kinase A, OTTHUMP00000174772
Protein Accession Number:
NP_937818.1,NP_000260.1
Protein Gi Number:
127981
Ncbi Gene Id Number:
4830
Background Reference 1:
Su Kim D, Choi YD, Moon M, Kang S, Lim JB, Kim KM, Park KM, Cho NH.Su Kim D, Choi YD, Moon M, Kang S, Lim JB, Kim KM, Park KM, Cho NH.Su Kim D, Choi YD, Moon M, Kang S, Lim JB, Kim KM, Park KM, Cho NH.Composite three-marker assay for early detection of kidney cancer.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-626

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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