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Western blot analysis in A549 cell line and human placenta lysates (35ug/lane).
Western blot analysis in A549 cell line and human placenta lysates (35ug/lane).

NME1 Antibody

CATALOG NUMBER: 58-191

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This NME1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-57 amino acids from the Central region of human NME1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
17 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NME1
Additional Names:
Nucleoside diphosphate kinase A, NDK A, NDP kinase A, Granzyme A-activated DNase, GAAD, Metastasis inhibition factor nm23, NM23-H1, Tumor metastatic process-associated protein, NME1, NDPKA, NM23
Protein Accession Number:
P15531
Protein Gi Number:
127981
Ncbi Gene Id Number:
4830
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene (NME1) was identified because of its reducedmRNA transcript levels in highly metastatic cells. Nucleosidediphosphate kinase (NDK) exists as a hexamer composed of 'A'(encoded by this gene) and 'B' (encoded by NME2) isoforms.Mutations in this gene have been identified in aggressiveneuroblastomas. Two transcript variants encoding different isoformshave been found for this gene. Co-transcription of this gene andthe neighboring downstream gene (NME2) generatesnaturally-occurring transcripts (NME1-NME2), which encodes a fusionprotein comprised of sequence sharing identity with each individualgene product.
Background Reference 1:
Boissan, M., et al. Cancer Res. 70(19):7710-7722(2010)
Background Reference 2:
Wang, P.H., et al. Gynecol. Oncol. 119(1):70-75(2010)
Background Reference 3:
Conery, A.R., et al. Proc. Natl. Acad. Sci. U.S.A. 107(35):15461-15466(2010)
Background Reference 4:
Wang, Z., et al. Med. Sci. Monit. 16 (8), CR357-CR364 (2010) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-191

List Size:
400 ul

List Price:

$495.00

Datasheet
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