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Antibody used in WB on Human HepG2 at 0.25 ug/ml.
Antibody used in WB on Human HepG2 at 0.25 ug/ml.

NMNAT1 Antibody

CATALOG NUMBER: 30-467

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NMNAT1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
NMNAT1 antibody can be used for detection of NMNAT1 by ELISA at 1:12500. NMNAT1 antibody can be used for detection of NMNAT1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
32 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store NMNAT1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
NMNAT1
Additional Names:
NMNAT1, NMNAT, PNAT-1, PNAT1, LCA9
Protein Accession Number:
NP_073624
Protein Gi Number:
20070321
Ncbi Gene Id Number:
64802
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The coenzyme NAD and its derivatives are involved in hundreds of metabolic redox reactions and are utilized in protein ADP-ribosylation, histone deacetylation, and in some Ca (2+) signaling pathways. NMNAT (EC 2.7.7.1) is a central enzyme in NAD biosynthesis, catalyzing the condensation of nicotinamide mononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) with the AMP moiety of ATP to form NAD or NaAD.The coenzyme NAD and its derivatives are involved in hundreds of metabolic redox reactions and are utilized in protein ADP-ribosylation, histone deacetylation, and in some Ca (2+) signaling pathways. NMNAT (EC 2.7.7.1) is a central enzyme in NAD biosynthesis, catalyzing the condensation of nicotinamide mononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) with the AMP moiety of ATP to form NAD or NaAD (Zhang et al., 2003 [PubMed 12574164]).
Background Reference 1:
Sorci, L., (2007) Biochemistry 46 (16), 4912-4922.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

30-467

List Size:
100 ul

List Price:

$473.00

Datasheet
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