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42-336 (0.5ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-336 (0.5ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Nmnat3 Antibody

CATALOG NUMBER: 42-336

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Immunogen:
The immunogen for this antibody is: C-KALGQGQSVKYLLPE
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 28kDa band observed in Mouse Heart lysates (calculated MW of 27.7kDa according to NP_653116.1). Recommended concentration: 0.5-2ug/ml.An additional strong band of unknown identity was also consistently observed at 75kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Specificity:
This antibody is expected to recognize both reported isoforms (NP_835471.1; NP_001186976.1).
Positive Control 1:
Cat. No. 1401 - Mouse Heart Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
Nmnat3
Additional Names:
Nmnat3, nicotinamide nucleotide adenylyltransferase 3, 4933408N02Rik, MGC102340, PNAT3, NMN adenylyltransferase 3, OTTMUSP00000025890, OTTMUSP00000025891, naMN adenylyltransferase 1, nicotinamide mononucleotide adenylyltransferase 3, nicotinate-nucleotide adenylyltransferase 1
Protein Accession Number:
NP_653116.1
Protein Gi Number:
21362329
Ncbi Gene Id Number:
74080(mouse);

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-336

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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