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Western Blot at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: human liver lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of NNMT using rabbit polyclonal NNMT Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NNMT gene (Lane 2).
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with NNMT antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western Blot at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: human liver lysate Lysates/proteins at 20 ug per lane.

NNMT Antibody

CATALOG NUMBER: 55-106

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This NNMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-106 amino acids from the Central region of human NNMT.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
30 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NNMT
Additional Names:
Nicotinamide N-methyltransferase, NNMT
Protein Accession Number:
P40261
Protein Gi Number:
730163
Ncbi Gene Id Number:
4837
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. NNMT is the protein responsible for this enzymatic activity, which uses S-adenosyl methionine as the methyl donor.
Background Reference 1:
Xu,J., Thyroid 16 (2), 151-160 (2006)
Background Reference 2:
Roessler,M., Clin. Cancer Res. 11 (18), 6550-6557 (2005)
Background Reference 3:
Souto,J.C., Am. J. Hum. Genet. 76 (6), 925-933 (2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-106

List Size:
400 ul

List Price:

$495.00

Datasheet
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