NNT-1 Antibody

CATALOG NUMBER: 38-130

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hNNT-1/BCSF-3. Human NNT-1/BCSF-3 specific antibody was purified by affinity chromatography employing immobilized hNNT-1/BCSF-3 matrix.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ELISA:
Indirect:
To detect hNNT-1 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNNT-1.
Sandwich
To detect hNNT-1by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human NNT-1 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNNT-1.
Western Blot:
To detect hNNT-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNNT-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
NNT-1 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
CLCF1
Additional Names:
CLC, NR6, BSF3, NNT1, BSF-3, CISS2, NNT-1, CLC, Cardiotrophin-like cytokine factor 1, B-cell-stimulating factor 3
Protein Accession Number:
Q9UBD9
Protein Gi Number:
56404673
Ncbi Gene Id Number:
23529
User Note:
Centrifuge vial prior to opening.
Background:
NNT1 belongs to the interleukin 6 family of cytokines, which are involved in cell signaling through phosphorylation of gp130. IL6 family members share similarity in gene structure and have a 4 helix bundle in their protein structure.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

38-130

List Size:
0.1 mg

List Price:

$408.00

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