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<strong> Figure 1 WB Validation in EL4 Cells </strong><br>Loading: 15 μg of lysate Antibodies: NOD4, 5951, 1 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Immunohistochemistry Validation of NOD4 in Human Kidney</strong><br> Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NOD4 antibody (5951) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 3 Immunohistochemistry Validation of NOD4 in Mouse Brain</strong><br> Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NOD4 antibody (5951) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 4 Immunohistochemistry Validation of NOD4 in Rat Kidney</strong><br> Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-NOD4 antibody (5951) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Figure 1 WB Validation in EL4 Cells
Loading: 15 μg of lysate Antibodies: NOD4, 5951, 1 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Trial Size

NOD4 Antibody

CATALOG NUMBER: 5951

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Anti-NOD4 antibody (5951) was raised against a peptide corresponding to 18 amino acid synthetic peptide near the amino terminus of human NOD4.

The immunogen is located within 20-70 amino acids of NOD4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
WB: 1-2 μg/mL; IHC-P: 1-2 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human, mouse, and rat samples. All other applications and species not yet tested.
Specificity:
NOD4 antibody is predicted to not cross-react with other members of the NOD family of proteins.
Predicted Molecular Weight:
Predicted: 205/146/79 kD

Observed: 205/146/79 kD
Purification:
NOD4 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
NOD4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
NOD4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NLRC5
Additional Names:
NOD4 Antibody: NOD4, NOD27, CLR16.1, NOD4, Protein NLRC5, Nucleotide-binding oligomerization domain protein 27
Protein Accession Number:
NP_115582
Protein Gi Number:
116268109
Ncbi Gene Id Number:
84166
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NOD4 Antibody: NOD4 is a member of the NOD (nucleotide-binding oligomerization domain) family, a group of proteins that are involved in innate immune defense. NOD4 contains a CARD-like domain, a central NOD domain and a large LRR region. NOD4, an IFN-gamma-inducible nuclear protein, plays a role in homeostatic control of innate immunity and in antiviral defense mechanisms. As a key negative regulator of NF-κB and type I interferon signaling, NOD4 may be a useful target for manipulating immune responses against infectious or inflammation-associated diseases, including cancer.
Background Reference 1:
Kufer TA, Banks DJ, and Philpott DJ. Innate immune sensing of microbes by Nod proteins. Ann. NY Acad. Sci. 2006; 1072:19-27.
Background Reference 2:
Benko S, Magalhaes JG, Philpott DJ, et al. NLRC5 limits the activation of inflammatory pathways. J. Immunol. 2010; 185:1681-91.
Background Reference 3:
Meissner TB, Li A, Biswas A, et al. NLR family member NLRC5 is a transcriptional regulator of MHC class I genes. Proc. Natl. Acad. Sci. USA 2010; 107:13794-9.
Background Reference 4:
Cui J, Zhu L, Xia X, et al. NLRC5 negatively regulates the NF-kappaB and type I interferon signaling pathways. Cell 2010; 141:483-96.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5951

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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