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Western blot analysis of NogoA in human brain tissue lysate with NogoA antibody at (A) 0.5 and (B) 1 μg/mL.
Immunohistochemistry of NogoA in mouse brain tissue with NogoA antibody at 2.5 μg/mL.
Immunofluorescence of NogoA in Mouse Brain tissue with NogoA antibody at 20 μg/mL.
Immunohistochemistry of NogoA in mouse brain tissue with NogoA antibody at 5 μg/ml.
Immunohistochemistry of NogoA in mouse brain tissue with NogoA Antibody at 5 μg/mL.
Immunofluorescence of NogoA in mouse brain tissue with NogoA Antibody at 20 μg/mL.<br><br>Green: NogoA antibody (4089) <br> Red: Phylloidin staining<br> Blue: DAPI staining
Western blot analysis of NogoA in human brain tissue lysate with NogoA antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

NogoA Antibody

CATALOG NUMBER: 4089

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
NogoA antibody was raised against a 19 amino acid synthetic peptide from near the center of human NogoA.
The immunogen is located within amino acids 200 - 250 of NogoA.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, IHC-P, WB
Application Note:
NogoA antibody can be used for detection of NogoA by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
At least five isoforms of Nogo are known to exist; this antibody is specific for NogoA and NogoE.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Positive Control 2:
Cat. No. 1403 - Mouse Brain Tissue Lysate
Predicted Molecular Weight:
Predicted: 131 kDa
Observed: ~180 kDa
Purification:
NogoA Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
NogoA Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
NogoA antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RTN4
Additional Names:
NogoA Antibody: ASY, NSP, NOGO, NOGOC, RTN-X, NOGO-A, NSP-CL, Nogo-B, Nogo-C, RTN4-A, RTN4-C, RTN4-B1, RTN4-B2, NI220/250, Nbla00271, Nbla10545, KIAA0886, My043, SP1507, Reticulon-4, Foocen, Nogo protein
Protein Accession Number:
NP_065393
Protein Gi Number:
24431935
Ncbi Gene Id Number:
57142
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NogoA Antibody: NogoA is a member of a family of integral membrane proteins termed reticulons that are thought to be involved in numerous disorders including neurodegenerative diseases. Reticulon proteins are known to regulate many cellular processes and interact with multiple proteins and receptors such as BACE. NogoA was initially identified as a myelin-associated neurite outgrowth inhibitor. It is highly expressed in oligodendrocytes in the white matter of the CNS; blocking its activity with antibodies or other factors results in improved axon regrowth and functional recovery in experimental CNS lesion models. NogoA has also been suggested to play a role in neurodegenerative diseases such as Amyotrophic lateral sclerosis, in which case NogoA is found at elevated levels in postmortem muscular samples, and multiple sclerosis (MS), in which case autoantibodies to NogoA have been found in serum and cerebrospinal fluid in MS patients.
Background Reference 1:
Yan R, ShiQ, Hu X, et al. Reticulon proteins: emerging players in neurodegenerative diseases. Cell. Mol. Life Sci. 2006; 63:877-889.
Background Reference 2:
Chen MS, Huber AB, van der Haar ME, et al. Nogo-A is a myelin-associated neurite outgrowth inhibitor and an antigen for monoclonal antibody IN-1. Nature 2000; 403:434-9.
Background Reference 3:
Schweigreiter R and Bandtlow CE. Nogo in the injured spinal cord. J. Neurotrauma 2006; 3-4:384-96.
Background Reference 4:
Dupuis L, Gonzalez de Aguilar JL, di Scala F, et al. Nogo provides a molecular marker for diagnosis of amyloid lateral sclerosis. Neurobiol. Dis. 2002; 10:358-65.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4089

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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