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Antibody used in WB on Human 721_B at 0.2-1 ug/ml.
Antibody used in WB on Human 721_B at 0.2-1 ug/ml.

NOL6 Antibody

CATALOG NUMBER: 25-660

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NOL6.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
NOL6 antibody can be used for detection of NOL6 by ELISA at 1:1562500. NOL6 antibody can be used for detection of NOL6 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
721_B Cell Lysate
Predicted Molecular Weight:
127 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store NOL6 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
NOL6
Additional Names:
NOL6, FLJ21959, MGC14896, MGC14921, MGC20838, NRAP, UTP22, bA311H10.1
Protein Accession Number:
NP_075068
Protein Gi Number:
18644728
Ncbi Gene Id Number:
65083
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NOL6 is a nucleolar RNA-associated protein that is highly conserved between species. RNase treatment of permeabilized cells indicates that the nucleolar localization is RNA dependent. Further studies suggest that the protein is associated with ribosome biogenesis through an interaction with pre-rRNA primary transcripts. The nucleolus is a dense subnuclear membraneless organelle that assembles around clusters of rRNA genes and functions in ribosome biogenesis. This gene encodes a nucleolar RNA-associated protein that is highly conserved between species. RNase treatment of permeabilized cells indicates that the nucleolar localization is RNA dependent. Further studies suggest that the protein is associated with ribosome biogenesis through an interaction with pre-rRNA primary transcripts. Alternative splicing has been observed at this locus and two splice variants encoding distinct isoforms have been identified.
Background Reference 1:
Nousiainen, M., (2006) Proc. Natl. Acad. Sci. U.S.A. 103 (14), 5391-5396.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

25-660

List Size:
100 ul

List Price:

$519.00

Datasheet
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