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45-976 (0.1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-976 (3.75ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-976 (0.1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

NONO Antibody

CATALOG NUMBER: 45-976

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog
Immunogen:
The immunogen for this antibody is: C-NRAAPGAEFAPNK
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 60kDa band observed in Mouse Brain lysates (calculated MW of 54.2kDa according to Human NP_031389.3 and 54.5kDa according to Mouse NP_075633.2). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:Paraffin embedded Human Spleen and Testis. Recommended concentration: 3.75ug/ml.
Positive Control 1:
Cat. No. 1403 - Mouse Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
NONO
Additional Names:
NONO, P54NRB , non-POU domain containing, octamer-binding , HGNC:7871, NMT55, NRB54, P54, Nuclear RNA-binding protein, 54-kD, non-POU-domain-containing, octamer-binding, non-Pou domain-containing octamer (ATGCAAAT) binding protein
Protein Accession Number:
NP_031389.3
Protein Gi Number:
34932414
Ncbi Gene Id Number:
4841
Background Reference 1:
Brown SA, Ripperger J, Kadener S, Fleury-Olela F, Vilbois F, Rosbash M, Schibler U. PERIOD1-associated proteins modulate the negative limb of the mammalian circadian oscillator. Science. 2005 Apr 29;308(5722):693-6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-976

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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