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Western blot analysis in Hela cell line lysates (35ug/lane).
NONO Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in Hela cell line lysates (35ug/lane).

NONO Antibody

CATALOG NUMBER: 57-268

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This NONO antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 45-74 amino acids from the N-terminal region of human NONO.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
54 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NONO
Additional Names:
Non-POU domain-containing octamer-binding protein, NonO protein, 54 kDa nuclear RNA- and DNA-binding protein, 55 kDa nuclear protein, DNA-binding p52/p100 complex, 52 kDa subunit, NMT55, p54(nrb), p54nrb, NONO, NRB54
Protein Accession Number:
Q15233
Protein Gi Number:
67469924
Ncbi Gene Id Number:
4841
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes an RNA-binding protein which playsvarious roles in the nucleus, including transcriptional regulationand RNA splicing. A rearrangement between this gene and thetranscription factor E3 gene has been observed in papillary renalcell carcinoma. Alternatively spliced transcript variants have beendescribed. Pseudogenes exist on Chromosomes 2 and 16. [provided byRefSeq].
Background Reference 1:
Marko, M., et al. Exp. Cell Res. 316(3):390-400(2010)
Background Reference 2:
Dong, X., et al. Mol. Endocrinol. 23(8):1147-1160(2009)
Background Reference 3:
Sikora, D., et al. Virology 390(1):71-78(2009)
Background Reference 4:
Clemson, C.M., et al. Mol. Cell 33(6):717-726(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-268

List Size:
400 ul

List Price:

$495.00

Datasheet
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