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Immunostaining of NOS1 neurons in cryosection of a perfusion-fixed (4% PFA) mouse hypothalamus. HRP-staining with Ni-DAB, after Biotin-SP-anti-goat (IgG) method.
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunostaining of NOS1 neurons in cryosection of a perfusion-fixed (4% PFA) mouse hypothalamus. HRP-staining with Ni-DAB, after Biotin-SP-anti-goat (IgG) method.

NOS1 Antibody

CATALOG NUMBER: 45-098

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, IF, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-ESKKDTDEVFSS
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Immunohistochemistry:Cryosections of Human and Mouse Hypothalamus. Optimal dilution: 1:3000.Immunofluorescence: Expression of the protein seen in the nucleus and plasma membrane of HeLa cells and in the nucleus and cytoplasm of U2OS cells. Recommended concentration: 10ug/ml.Flow Cytometry: Flow cytometric analysis of Kelly cells. Recommended concentration: 10ug/ml.
Positive Control 1:
Cat. No. 1375 - Human Skeletal Muscle Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
NOS1
Additional Names:
NOS1, nitric oxide synthase 1 (neuronal), NOS, neuronal nitric oxide synthase, PnNOS, penile neuronal nitric oxide synthase, penile neuronal NOS, IHPS1, nNOS, nitric oxide synthase 1, neuronal
Protein Accession Number:
NP_000611.1,NP_001191147.1,NP_001191142.1
Protein Gi Number:
10835173
Ncbi Gene Id Number:
4842
Background Reference 1:
Fujisawa H, Ogura T, Kurashima Y, Yokoyama T, Yamashita J, Esumi H. Expression of two types of nitric oxide synthase mRNA in human neuroblastoma cell lines. J Neurochem 1994 Jul;63(1):140-5

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-098

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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