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Western blot analysis of lysate from MCF-7 cell line, using NOS1AP Antibody at 1:1000 at each lane.
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of lysate from MCF-7 cell line, using NOS1AP Antibody at 1:1000 at each lane.

NOS1AP Antibody

CATALOG NUMBER: 55-115

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This NOS1AP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 437-466 amino acids from the Central region of human NOS1AP.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
56 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NOS1AP
Additional Names:
Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein, C-terminal PDZ ligand of neuronal nitric oxide synthase protein, Nitric oxide synthase 1 adaptor protein, NOS1AP, CAPON, KIAA0464
Protein Accession Number:
O75052
Ncbi Gene Id Number:
9722
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a cytosolic protein that binds to thesignaling molecule, neuronal nitric oxide synthase (nNOS). Thisprotein has a C-terminal PDZ-binding domain that mediatesinteractions with nNOS and an N-terminal phosphotyrosine binding(PTB) domain that binds to the small monomeric G protein, Dexras1.Studies of the related mouse and rat proteins have shown that thisprotein functions as an adapter protein linking nNOS to specifictargets, such as Dexras1 and the synapsins. Alternative splicingresults in multiple transcript variants encoding differentisoforms.
Background Reference 1:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 2:
Lu, J., et al. Diabet. Med. 27(9):1074-1079(2010)
Background Reference 3:
Husted, J.A., et al. Schizophr. Res. 121 (1-3), 187-192 (2010) :
Background Reference 4:
Tomas, M., et al. J. Am. Coll. Cardiol. 55(24):2745-2752(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-115

List Size:
400 ul

List Price:

$495.00

Datasheet
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