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Human NOTCH1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human DLL4, His Tag at 5 ug/mL (100 uL/well) can bind Human NOTCH1, Fc Tag with a linear range of 1-8 ng/mL (QC tested).
Human NOTCH1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

NOTCH1 Recombinant Protein

CATALOG NUMBER: 11-162

Tested Applications:
ELISA, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Ala 19 - Gln 526
Fusion Tag:
Fc Tag
Tested Applications:
ELISA, WB
Application Note:
This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 80.0 kDa. The protein migrates as 90-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Predicted Molecular Weight:
80.0 kDa
Purity:
>95% as determined by SDS-PAGE.
physical-state:
Lyophilized
Buffer:
PBS, pH7.4
Storage Conditions:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
NOTCH1
Additional Names:
NOTCH1,Notch 1,hN1,TAN1
Protein Accession Number:
NP_060087.3
Ncbi Gene Id Number:
4851
Background:
NOTCH1 Interacts with DNER, DTX1, DTX2 and RBPJ/RBPSUH. Also interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH1. The NOTCH1 intracellular domain interacts with SNW1; the interaction involves multimerized NOTCH1 NICD and is implicated in a formation of an intermediate preactivation complex which associates with DNA-bound CBF-1/RBPJ. The activated membrane-bound form interacts with AAK1 which promotes NOTCH1 stabilization. Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia.
Background Reference 1:
Wu L, et.al. Nat Genet. 2000. 26(4):484-9.
Background Reference 2:
Wu L, et.al. Mol Cell Biol. 2002. 22(21):7688-700.
Background Reference 3:
Zhou S, et al. Mol Cell Biol. 2000. 20(7):2400-10.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

11-162

List Size:
0.1 mg

List Price:

$577.00

Datasheet
Shipping Info

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