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45-978 (4ug/ml) staining of paraffin embedded Human Hippocampus. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
45-978 (5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.
45-978 (4ug/ml) staining of paraffin embedded Human Hippocampus. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

NOTCH3 Antibody

CATALOG NUMBER: 45-978

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-QLGPQPEVTPKRQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave approx 60+70kDa bands in human brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 244kDa according to NP_000426.2. The 60+70kDa bands were successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:In paraffin embedded Human Hippocampus shows select nuclear staining and weaker staining in different comparments of the neuronal cell bodies. Recommended concentration: 4-6ug/ml. Paraffin embedded Human Placenta. Recommended concentration: 5ug/ml.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
NOTCH3
Additional Names:
NOTCH3, Notch homolog 3 (Drosophila), HGNC:7883, CADASIL, CASIL , Notch (Drosophila) homolog 3, Notch homolog 3
Protein Accession Number:
NP_000426.2
Protein Gi Number:
4557799
Ncbi Gene Id Number:
4854
Background Reference 1:
Dang L, Fan X, Chaudhry A, Wang M, Gaiano N, Eberhart CG. Notch3 signaling initiates choroid plexus tumor formation. Oncogene. 2005 Sep 26;

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-978

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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