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Western blot analysis of NOTUM in human kidney tissue lysate with NOTUM antibody at (A) 0.5 and (B) 1 μg/mL.
Immunohistochemistry of NOTUM in human brain tissue with NOTUM antibody at 2.5 μg/mL.
Immunofluorescence of NOTUM in Mouse Kidney cells with NOTUM antibody at 20 μg/mL.
Western blot analysis of NOTUM in human kidney tissue lysate with NOTUM antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

NOTUM Antibody

CATALOG NUMBER: 5291

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
NOTUM antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human NOTUM.
The immunogen is located within amino acids 90 - 140 of NOTUM.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
NOTUM antibody can be used for detection of NOTUM by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1305 - Human Kidney Tissue Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
NOTUM Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
NOTUM Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
NOTUM antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NOTUM
Additional Names:
NOTUM Antibody: Protein notum homolog
Protein Accession Number:
NP_848588
Protein Gi Number:
194394139
Ncbi Gene Id Number:
147111
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NOTUM Antibody: In Drosophila, the notum gene is regulated by the Wingless pathway and encodes a secreted hydrolase that modifies heparan sulfate proteoglycans. The mammalian homolog has been shown to be able to cleave glypicans and can release GPI-anchored proteins from the mammalian cell surface. Like the Drosophilia NOTUM, the mammalian protein can act as a negative regulator of the Wnt signaling pathway. NOTUM is expressed at a low level in most mammalian tissues, although it is overexpressed in a subset of human hepatocellular carcinomas. Its transcription is regulated by beta-catenin/TCF and is a target of the Wnt signaling pathway, forming a negative feedback loop that regulates the expression and activity of the Wnt pathway.
Background Reference 1:
Giraldez AJ, Copley RR, and Cohen SM. HSPG modification by the secreted enzyme Notum shapes the wingless morphogen gradient. Dev. Cell2002; 667-76.
Background Reference 2:
Traister A, Shi W, and Filmus J. Mammalian Notum induces the release of glypicans and other GPI-anchored proteins from the cell surface. Biochem. J.2008; 410:503-11.
Background Reference 3:
Torisu Y, Watanabe A, Nonaka A, et al. Human homolog of NOTUM, overexpressed in hepatocellular carcinoma, is regulated transcriptionally by b-catenin/TCF. Cancer Sci.2008; 6:1139-46.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5291

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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