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46-694 (0.01ug/ml) staining of Human Breast Cancer lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
HEK293 overexpressing NOVA1 and probed with 46-694 (mock transfection in first lane).
46-694 (5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-694 (5ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-694 (0.01ug/ml) staining of Human Breast Cancer lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

NOVA1 Antibody

CATALOG NUMBER: 46-694

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-REMPQNVAKTEPVS
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 50-55kDa band observed in Human Breast Cancer lysates (calculated MW of 51.7kDa according to NP_002506.2). In transfected HEK293 transiently expressing NOVA1 a band of approx. 52kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.01-0.03ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cortex) and Heart. Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognise all three reported isoforms (NP_002506.2 ; NP_006480.2 ; NP_006482.1)
Positive Control 1:
Cat. No. 1323 - Human Breast Tumor Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
NOVA1
Additional Names:
NOVA1, neuro-oncological ventral antigen 1, Nova-1, paraneoplastic Ri antigen, ventral neuron-specific protein 1
Protein Accession Number:
NP_002506.2,NP_006480.2,NP_006482.1
Protein Gi Number:
56549653
Ncbi Gene Id Number:
4857
Background Reference 1:
Jelen N, Ule J, Zivin M, Darnell RB. Evolution of Nova-dependent splicing regulation in the brain. PLoS Genet. 2007 Oct 12;3(10):1838-47. Epub 2007 Aug 27.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-694

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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