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42-316 (0.1ug/ml) staining of lysate of cell line colorectal adenocarcinoma (A) and HeLa (B) (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-316 (7.5ug/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
42-316 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
42-316 (0.1ug/ml) staining of lysate of cell line colorectal adenocarcinoma (A) and HeLa (B) (35ug protein in RIPA buffer). Detected by chemiluminescence.

NOX1 Antibody

CATALOG NUMBER: 42-316

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-DKATDIVTGLKQK
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 55kDa band observed in lysates of cell line epithelial colorectal adenocarcinoma and HeLa (calculated MW of 59kDa according to NP_39249.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 7.5ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_008983.2; NP_39249.1).
Positive Control 1:
Cat. No. 1320 - Human Colon Tissue Lysate
Positive Control 2:
Cat. No. 1411 - Mouse Colon Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
NOX1
Additional Names:
NOX1, NADPH oxidase 1, GP91-2, MOX1, NOH-1, NOH1, MOX-1, NADH/NADPH mitogenic oxidase subunit P65-MOX, NADPH oxidase 1 variant NOH-1L, NADPH oxidase homolog-1, NOX-1, mitogenic oxidase (pyridine nucleotide-dependent superoxide-generating), mitogenic oxidase 1
Protein Accession Number:
NP_008983.2,NP_39249.1
Protein Gi Number:
148536873
Ncbi Gene Id Number:
27035

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-316

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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