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Western blot analysis of NOX2 in rat brain tissue lysate with NOX2 antibody at 1 μg/ml.
Immunohistochemistry of NOX2 in rat brain tissue with NOX2 antibody at 5 μg/ml.
Immunofluorescence of NOX2 in rat brain tissue with NOX2 antibody at 20 μg/ml.
Immunohistochemistry of NOX2 in rat brain tissue cells with NOX2 antibody at 2 μg/ml.
Immunofluorescence of NOX2 in rat brain tissue with NOX2 antibody at 20 μg/ml.<br><br>Green: NOX2 Antibody (7923) <br> Blue: DAPI staining
Western blot analysis of NOX2 in rat brain tissue lysate with NOX2 antibody at 1 μg/ml.
Trial Size

NOX2 Antibody

CATALOG NUMBER: 7923

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (87%), Bovine: (87%)
Immunogen:
Rabbit polyclonal NOX2 antibody was raised against a 15 amino acid peptide near the amino terminus of human NOX2.
The immunogen is located within amino acids 140 - 190 of NOX2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
NOX2 antibody can be used for detection of NOX2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Specificity:
NOX2 antibody is human, mouse and rat reactive. At least two isoforms are known to exist; this antibody will detect both isoforms. NOX2 is predicted to not cross-react with other NOX proteins.
Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Predicted Molecular Weight:
Predicted: 59, 63 kDa
Observed: 58 kDa
Purification:
NOX2 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
NOX2 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
NOX2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
CYBB
Additional Names:
NOX2 Antibody: CGD, NOX2, AMCBX2, GP91-1, GP91PHOX, p91-PHOX, GP91-PHOX, Cytochrome b-245 heavy chain, CGD91-phox, Cytochrome b558 subunit beta
Protein Accession Number:
NP_000388
Protein Gi Number:
6996021
Ncbi Gene Id Number:
1536
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The NOX family of NAPDH oxidases is comprised of seven transmembrane proteins that oxidize intracellular NAPDH/NADH, causing electron transport across the membrane and the reduction of molecular oxygen to superoxide (1). NOX2, also known as cytochrome b beta (CYBB) is one of two proteins that make up Cytochrome b-245, thought to be a primary component of the microbicidal oxidase system of phagocytes. NOX2 deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD) (2). Activation of the NOX2 enzyme complex in microglia is thought to be neurotoxic and may play a role in Alzheimer's and Parkinson's disease (3).
Background Reference 1:
Bedard K and Krause KH. The Nox family of ROS-generating NAPDH oxidases: physiology and pathophysiology. Physiol. Rev. 2007; 87:245-313.
Background Reference 2:
Segal AW. Cytochrome b-245 and its involvement in the molecular pathology of chronic granulomatous disease. Hematol. Oncol. North Am. 1988; 2:213-23.
Background Reference 3:
Surace MJ and Block ML. Targeting microglia-mediated neurotoxicity: the potential of NOX2 inhibitors. Cell Mol. Life Sci. 2012; 69:2409-27.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7923

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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